PBP 4 Mediates High-Level Resistance to New-Generation Cephalosporins in Staphylococcus aureus

MupB, a new high-level mupirocin resistance mechanism in Staphylococcus aureus.

Mupirocin is a topical antibiotic used for the treatment of skin infections and the eradication of methicillin-resistant Staphylococcus aureus carriage. It inhibits bacterial protein synthesis by interfering with isoleucyl-tRNA synthetase activity. High-level mupirocin resistance (MIC of ≥ 512 μg/ml) is mediated by the expression of mupA (ileS2), which encodes an alternate isoleucyl-tRNA synthe...

Vancomycin Resistance in Staphylococcus aureus Strains

Whole-Genome Sequencing of Methicillin-Resistant Staphylococcus aureus Resistant to Fifth-Generation Cephalosporins Reveals Potential Non-mecA Mechanisms of Resistance.

Fifth-generation cephalosporins, ceftobiprole and ceftaroline, are promising drugs for treatment of bacterial infections from methicillin-resistant Staphylococcus aureus (MRSA). These antibiotics are able to bind native PBP2a, the penicillin-binding protein encoded by the mecA resistance determinant that mediates broad class resistance to nearly all other beta-lactam antibiotics, at clinically ...

Transient loss of high-level mupirocin resistance in Staphylococcus aureus due to MupA polymorphism.

Spontaneous loss of MupA-mediated high-level mupirocin resistance was observed in Staphylococcus aureus, although the isolate gave a PCR-positive test result for mupA. Sequencing of the mupA gene identified a single base-pair deletion that resulted in a frameshift mutation and loss of functional protein. Reversion to the wild-type allele and restoration of high-level resistance occurred with hi...

Mutations to sulfonamide resistance in Staphylococcus aureus.

T WO general hypotheses have been postulated to explain the origin of drug-resistant strains of bacteria. These hfiotheses are: (I) Resistant cells arise by mutation and are selected when grown in the presence of drugs. (2) Resistance is the result of a specific adaptation of bacteria in response to inhibiting agents. Resistance of Escherickia coli to bacteriophage (LTJRIA and DELBR~CK, 1943) r...